Relative efficacy of egg albumin as blocking agent in dot immunobinding assay for detection of group B coxsackieviruses.
Indian J Exp Biol
;
1994 Sep; 32(9): 659-61
Article
in English
| IMSEAR
| ID: sea-57038
ABSTRACT
Non specific binding (NSB) is an important factor affecting sensitivity and specificity in dot immunobinding assay (DIA). Several blocking agents e.g. egg albumin, casein, gelatin, milk powder and goat serum were evaluated for their relative efficacy vis-a-vis bovine serum albumin (BSA) for DIA system purported for detection of group B coxsackieviruses (CVB). The results suggest that egg albumin (5%) which is economical and readily available may act as an effective blocking agent in DIA system.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Immunoblotting
/
Ovalbumin
/
Enterovirus B, Human
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1994
Type:
Article
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