Concentration of cations in different parts of male reproductive system and their influence on in vitro sperm motility in lizard, Mabuya carinata Schneider.

ABSTRACT

Concentrations of mono- and divalent cations in the testis, three different regions of the epididymis and the vas deferens of lizard, M. carinata were determined. The concentrations of Na+, K+ and Ca2+ increased gradually from testis to vas deferens. However, no significant variation in the levels of Mg2+ was observed between testis and anterior epididymis, and between posterior epididymis and vas deferens. Further, in an in vitro sperm motility study, wherein different concentrations of the cations were tested, the maximum forward progressive motility of posterior epididymal spermatozoa was observed following treatment with 140 mMNaCl, 25 mM KCl, and 50 mM CaCl2 whereas, treatment with magnesium brought about a decrease in sperm motility. The results indicate that Na+, K+, and Ca2+ have stimulatory effect at an optimum dose, whereas Mg2+ has an inhibitory effect even at very low concentration, on sperm motility in the lizard M carinata.