Adoptive immunotherapy of murine lymphosarcoma: comparative study using allo-immune or tumor-immune T cells.
Indian J Exp Biol
;
1997 Jun; 35(6): 581-8
Article
in English
| IMSEAR
| ID: sea-57199
ABSTRACT
The antitumor effect of allosensitization with lymphocytes and skin graft of DBA/2 mice was evaluated using immunogeneic, transplantable Lymphosarcoma (LS-A) syngeneic to Swiss mice. A dose dependent tumor inhibitory effect in terms of tumor free mice was observed in mice sensitized i.p. with lymphocyte doses between 10-100 million per animal. Sensitization with allogeneic primary skin graft was more effective than lymphocyte immunization. The antitumor immunity could be adoptively transferred in syngeneic Swiss mice using either allo-immune or tumor-immune T cells. Analysis of T cell phenotypes using monoclonal antibodies against cell surface markers CD4 and CD8, indicated absolute dependence on the CD4+ T cells subset in tumor cure in case of allo-immune as well as tumor-immune T cells. CD8+ T cell subset was found essential only in case of allo-immune T cell therapy. Immunosuppression of mice with whole body gamma irradiation (4 Gy), 6 hr before transfer of allo-immune or tumor-immune T cells did not abrogate the therapeutic ability of allo-immune or tumor-immune T cells. Our results suggest that allosensitization could be an effective method of generating effector lymphocyte populations that might be used to treat tumors that exhibit detectable immunogenecity.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Lymphoma, Non-Hodgkin
/
T-Lymphocytes
/
Immunotherapy, Adoptive
/
Isoantibodies
/
Animals
/
Mice
/
Mice, Inbred DBA
Language:
English
Journal:
Indian J Exp Biol
Year:
1997
Type:
Article
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