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Polymorphism in Alnus based Frankia of Darjeeling.
Indian J Exp Biol ; 2005 Sep; 43(9): 813-6
Article in English | IMSEAR | ID: sea-57260
ABSTRACT
DNA samples extracted from the root nodules of Alnus nepalensis, collected from 10 different locations of Darjeeling hills, were used to assess the genetic diversity of Frankia. The DNA samples from the nodules of naturally growing plants were used as templates in PCR, targeting different genomic regions of Frankia, namely distal, middle and proximal parts of 16S rRNA gene and nifH-D IGS region with locus specific primers. The PCR products were digested with a number of frequent (4-base) cutter restriction endonucleases. Bands were scored as present (1) or absent (0) and the clustering was done using NTSYSpc. Distinct polymorphism was found among the nodules collected from different parts of the region and those of same geographic area. These results demonstrate that genetic diversity is indeed present among the naturally occurring Frankia of Darjeeling, India.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Phylogeny / Polymorphism, Genetic / Genetic Variation / Polymorphism, Restriction Fragment Length / RNA, Bacterial / RNA, Ribosomal, 16S / Cluster Analysis / Polymerase Chain Reaction / DNA Primers / Alnus Language: English Journal: Indian J Exp Biol Year: 2005 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Phylogeny / Polymorphism, Genetic / Genetic Variation / Polymorphism, Restriction Fragment Length / RNA, Bacterial / RNA, Ribosomal, 16S / Cluster Analysis / Polymerase Chain Reaction / DNA Primers / Alnus Language: English Journal: Indian J Exp Biol Year: 2005 Type: Article