Nutritional regulation of actinomycin-D production by a new isolate of Streptomyces sindenensis using statistical methods.
Indian J Exp Biol
;
2008 Feb; 46(2): 138-44
Article
in English
| IMSEAR
| ID: sea-57494
ABSTRACT
Production of actinomycin-D, by an isolate, S. sindenensis, was optimized by statistical methods. Fructose peptone and NaNO3 were found to be critical for antibiotic production. In the second step, their concentrations were optimized with Central Composite Design and Response Surface Methodology. Fructose, peptone and NaNO3 at 2.55, 0.309 and 0.114% respectively gave approximately 261% higher yield (289 mg/l). Cultivation in fermentor at 600 rpm agitation and 1.5 vvm aeration with optimized medium gave 3.56 folds higher yield (365 mg/l) as compared to the yields in shake flasks using normal production medium (80 mg/l).
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Streptomyces
/
Molecular Sequence Data
/
RNA, Ribosomal, 16S
/
Models, Statistical
/
Biomass
/
Bioreactors
/
Dactinomycin
/
Drug Industry
Type of study:
Risk factors
Language:
English
Journal:
Indian J Exp Biol
Year:
2008
Type:
Article
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