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Impaired cell motility in chronic myeloid leukemic granulocytes related to altered cytoskeletal pattern.
Indian J Exp Biol ; 2006 Mar; 44(3): 193-202
Article in English | IMSEAR | ID: sea-57532
ABSTRACT
The bactericidal activity of polymorphonuclear leucocyte (PMNL) against infection stimulates cytoskeletal changes accompanied with alteration in adhesion and locomotion. Microfilaments, the motile apparatus is known to regulate these changes by polymerization of monomeric G-actin to fibrous F-actin. PMNL from chronic myeloid leukemia (CML) patients have been reported to be defective in locomotion in response to synthetic peptide, n-formyl-methionyl-leucyl-phenylalanine (fMLP) but the mechanism leading to defective locomotion and their spatial reorganization remains unclear. Therefore, in order to study the cause of defective motility of PMNL from CML patients the spatial distribution and reorganization of microfilaments and microtubules in response to fMLP have been examined by transmission electron (TEM) and scanning electron microscopy (SEM). Under SEM, the PMNL-CML surface appeared smoother with reduced ruffling resulting in rounding off cells with lesser polarized morphology. Unstimulated PMNL from normal as well as CML subjects showed shorter and fewer microtubules and evenly distributed microfilaments as compared to fMLP stimulated PMNL. It is proposed that the cause of defective locomotion was due to reduced surface activity as a consequence of altered cytoskeletal configuration. This phenomenon seems to be related to impaired functional appendages and as a whole led to the defective cell motility and hence reduced chemotaxis in PMNL from CML patients.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Cytoskeleton / Humans / Microscopy, Electron, Scanning / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Cell Movement / Microscopy, Immunoelectron / Myosin Subfragments / Cell Death / Microscopy, Electron, Transmission / Gold Language: English Journal: Indian J Exp Biol Year: 2006 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Cytoskeleton / Humans / Microscopy, Electron, Scanning / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Cell Movement / Microscopy, Immunoelectron / Myosin Subfragments / Cell Death / Microscopy, Electron, Transmission / Gold Language: English Journal: Indian J Exp Biol Year: 2006 Type: Article