Regeneration from leaf protoplasts of Arabidopsis thaliana ecotype estland.
Indian J Exp Biol
;
2001 Jul; 39(7): 705-9
Article
in English
| IMSEAR
| ID: sea-57571
ABSTRACT
Protoplasts (2 x 10(7)/g fresh wt) were isolated from leaves of A. thaliana ecotype estland, with a viability of more than 90%. Protoplasts cultured in calcium alginate beads or layers showed division while culture in liquid or agarose beads failed to elicit any division. Effect of culture density showed highest frequency of division occurring at 5 x 10(5) while no division was seen when cultured at a density of 5 x 10(4). Culture in MS medium resulted in higher division frequency and better sustenance of microcolonies as compared to B5 medium. Under optimized conditions, macrocolonies were formed at a frequency of 1.8%. Shoot regeneration was seen in 50% of microcalli transferred to shoot induction medium for regeneration. Shoots were rooted and plantlets transferred to pots. The plants produced flowers and were fertile.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Protoplasts
/
Regeneration
/
Cell Division
/
Arabidopsis
/
Plant Leaves
Language:
English
Journal:
Indian J Exp Biol
Year:
2001
Type:
Article
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