In vitro clonal propagation of bird eye chilli (Capsicum frutescens Mill.).
Indian J Exp Biol
;
2004 Nov; 42(11): 1136-40
Article
in English
| IMSEAR
| ID: sea-58119
ABSTRACT
An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skoog (MS) medium containing 0.5-3.0 mgl(-1) of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg l(-1) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gl(-1) activated charcoal (AC) were used for shoot regeneration from both shoot tip and nodal explants. Shoot tip explants (100%) grew well on medium containing 1 mgl(-1) of kinetin and 1 mgl(-1) of IBA. Shoot proliferation (1-3) from nodal explants was effective on this medium. The regenerated shoots with 4-7 nodes had further growth upon sub-culturing onto kinetin (1 mgl(-1)) and IBA (1 mgl(-1)) and rooted simultaneously. The rooted plants were transferred to pots after hardening under controlled conditions. The survival percentage in pots was 80-90%.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Regeneration
/
Capsicum
/
Plant Shoots
/
Culture Media
Language:
English
Journal:
Indian J Exp Biol
Year:
2004
Type:
Article
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