Hormone-mediated positive and negative regulation of a silencer region containing laminin B2 promoter in astrocytes.
Indian J Exp Biol
;
1996 Oct; 34(10): 939-44
Article
in English
| IMSEAR
| ID: sea-58225
ABSTRACT
Primary astrocytes synthesize only B2 chain (200 Kda) of laminin, which is one of the major components of basement membranes in the central nervous system (CNS). In CNS, B2 laminin functions as a potent neurite growth factor. Laminin B2 promoter contain no TATA or CAAT boxes but is GC rich. By deletion analysis and transient transfection assays of B2 laminin promoter, we have identified a silencer-like activity in the upstream region of the promoter. Thyroid hormone, insulin and phorbol ester mediated the induction of the promoter activity. Induction was repressed by the treatment of astrocytes with synthetic glucocorticoid hormone, dexamethasone. Hormone-mediated regulation through specific positive and negative responsive elements in transactivation of this silencer-containing TATA-less laminin B2 gene has been postulated.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Humans
/
Transcriptional Activation
/
Genes, Regulator
/
Astrocytes
/
TATA Box
/
Laminin
/
Promoter Regions, Genetic
/
Hormones
/
Animals
Type of study:
Prognostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1996
Type:
Article
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