Role of PGrs and inhibitors in induction and control of somatic embryogenesis in Themeda quadrivalvis.
Indian J Exp Biol
;
2009 Mar; 47(3): 198-203
Article
in English
| IMSEAR
| ID: sea-58332
ABSTRACT
Somatic embryogenesis could be achieved in Themeda quadrivalvis (Linn.) O. Ktze -fodder grass species on MS medium supplemented with 2,4-D. Incorporation of putrescine in the medium stimulated embryogenesis, however its lower concentration stimulated production of non-regenerative callus. Other polyamines such as spermine and spermidine could not evoke similar response. Ascorbic acid used as antioxidant could not prevent browning in embryogenic cultures, however it stimulated embryogenesis. Inhibition of auxin polar transport by use of TIBA and HFCA reduced the embryogenic response significantly and produced distorted or abnormal embryos. Antiethylene substances such as AgNO3 and CoCl2 added in the medium adversely affected the process of embryogenesis and counteracting the stimulatory role of ethylene.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Growth Regulators
/
Ascorbic Acid
/
Seeds
/
Biogenic Polyamines
/
Germination
/
2,4-Dichlorophenoxyacetic Acid
/
Dose-Response Relationship, Drug
/
Indoleacetic Acids
Language:
English
Journal:
Indian J Exp Biol
Year:
2009
Type:
Article
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