Enhancement of radiation induced cell death in chicken B lymphocytes by withaferin A.
Indian J Exp Biol
;
2008 Jun; 46(6): 437-42
Article
in English
| IMSEAR
| ID: sea-58384
ABSTRACT
Withaferin A (WA), a plant withanolide, has shown significant radiosensitizing effect in vitro and in vivo. Inhibition of DNA repair has been suggested as a mechanism of radiosensitization by WA. To test this, the effect of withaferin A on survival of DT40 chicken B-lymphocyte cell line and its repair deficient single gene mutants Rad54-/-, Ku70-/- and double mutant Ku70-/- /Rad54-/- after irradiation was studied. Exponentially growing cells were treated for 1 hr with 5 microM WA and then exposed to different doses of X-rays. Cell survival was studied by clonogenic assay. WA significantly reduced survival of DT40, Ku70-/- and Ku70-/- /Rad54-/-, but not Rad54-/- cells, suggesting that WA enhances radiosensitivity by interfering with homologous repair, the major pathway of DSB repair in these cells. Inhibition of DNA repair is further indicated in a significant decrease in surviving fraction of DT40 cells by post-irradiation incubation with WA. This could have relevance to cancer radiotherapy.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Radiation-Sensitizing Agents
/
X-Rays
/
Nuclear Proteins
/
B-Lymphocytes
/
Cell Line
/
Chickens
/
Cell Death
/
DNA Repair
/
Ergosterol
/
Animals
Language:
English
Journal:
Indian J Exp Biol
Year:
2008
Type:
Article
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