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New Helicoverpa armigera Hbn cell line from larval hemocyte for baculovirus studies.
Indian J Exp Biol ; 2002 Jan; 40(1): 69-73
Article in English | IMSEAR | ID: sea-58483
ABSTRACT
A new cell line from the larval hemocytes of H. armigera was established in Grace's medium modified by adding lactalbumin hydrolysate and yeastolate (3.3g/l), and supplemented with fetal bovine serum (20%). The cell line was designated as NIV-HA-1195. The cell population at P-78 consisted mainly of epithelial-like cells (89.36%), fibroblast-like cells (8.31%) and giant cells (2.13%). The population doubling time was 96hr at P-8, 60hr at P-43. The chromosome number ranged from 45 to 200. The cell line is susceptible to the baculoviruses, Autographa californica nucleopolyhedrovirus (AcNPV), Spodoptera litura NPV and the homologous HaNPV. Isoenzyme profile and results of 16S rRNA heteroduplex analysis clearly indicated the species specificity of the new cell line.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Cell Division / Cell Line / Baculoviridae / Heteroduplex Analysis / Glucosephosphate Dehydrogenase / Isocitrate Dehydrogenase / L-Lactate Dehydrogenase / Larva / Animals / Malate Dehydrogenase Language: English Journal: Indian J Exp Biol Year: 2002 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Cell Division / Cell Line / Baculoviridae / Heteroduplex Analysis / Glucosephosphate Dehydrogenase / Isocitrate Dehydrogenase / L-Lactate Dehydrogenase / Larva / Animals / Malate Dehydrogenase Language: English Journal: Indian J Exp Biol Year: 2002 Type: Article