Detection of spores of Bacillus anthracis from environment using polymerase chain reaction.
Indian J Exp Biol
;
2003 Feb; 41(2): 177-80
Article
in English
| IMSEAR
| ID: sea-58515
ABSTRACT
A sensitive PCR based detection of Bacillus anthracis spores from environnment was standardized. Specific 1247bp amplicon could be detected with template concentration as low as 13 pg. Sensitivity was enhanced to 10 fold by nesting with second set of primers, forming 208bp amplicon. Extraction of DNA from spores purified from soil samples by aqueous polymer two-phase system followed by partial germination and freeze-thaw treatment yielded best results. Soil sample spiked with spores (8x10(2)/g of sample) could be detected with this method.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Spores, Bacterial
/
Bacillus anthracis
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Environment
/
Environmental Microbiology
/
Anthrax
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
2003
Type:
Article
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