Lytic effect of Bacillus subtilis elastase on gram-positive and negative bacteria.
Indian J Exp Biol
;
1992 May; 30(5): 380-3
Article
in English
| IMSEAR
| ID: sea-58536
ABSTRACT
Elastase of B. subtilis 6a caused lysis of freshly grown cells of Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, Salmonella typhi and Pseudomonas aeruginosa and Gram-positive (B. subtilis) bacteria. Heat killed and lyophilised Gram-positive and negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacillus subtilis
/
Bacterial Proteins
/
Bacteriolysis
/
Pancreatic Elastase
/
Gram-Negative Bacteria
/
Gram-Positive Bacteria
/
Hydrogen-Ion Concentration
Language:
English
Journal:
Indian J Exp Biol
Year:
1992
Type:
Article
Similar
MEDLINE
...
LILACS
LIS