Comparison of two colorimetric assays to determine viral infectivity in micro culture virus titration.
Indian J Exp Biol
;
1999 Dec; 37(12): 1223-6
Article
in English
| IMSEAR
| ID: sea-58690
ABSTRACT
Efficacy of two colorimetric assays, viz. MTT (3-4,5-dimethylthiazol-2-(yl-2,5-diphenyl tetrazolium bromide) and neutral red (NR) assays, performed by integrating them to micro culture virus titration (MCVT), was compared with the conventional MCVT method in terms of percentages of infectivity and 50% infectivity end points by employing Polio virus type-3 and Dengue virus type 4 as the candidate viruses. The results suggested that MTT assay has an edge over NR assay as well as conventional MCVT method. For the first time, NR assay has been successfully employed for the determination of virus infectivity titre.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Tetrazolium Salts
/
Thiazoles
/
Virology
/
Vero Cells
/
Humans
/
Cell Line
/
Chlorocebus aethiops
/
Colorimetry
/
Cytopathogenic Effect, Viral
/
Poliovirus
Type of study:
Diagnostic study
/
Evaluation studies
Language:
English
Journal:
Indian J Exp Biol
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS