Construction of chickpea genomic library.
Indian J Exp Biol
;
1989 Oct; 27(10): 858-60
Article
in English
| IMSEAR
| ID: sea-58991
ABSTRACT
For construction of chickpea genomic library, DNA was isolated, purified on CsCl gradient and size fractionated into 15-20 Kb fragments after restriction with Sau 3A. These fragments were ligated to phage lambda (EMBL-3) vector and the recombinant molecules packaged in vitro into viable phage particles. The recombinant phages were obtained as phages on a P2 lysogen of E. coli (Spi- selection) and amplified to establish a permanent library. This is the first report of the construction of chickpea genomic library.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plants, Medicinal
/
DNA
/
Genomic Library
/
Fabaceae
Language:
English
Journal:
Indian J Exp Biol
Year:
1989
Type:
Article
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