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Mode of utilization of amino acids as growth substrates by Azospirillum brasilense.
Indian J Exp Biol ; 2005 Dec; 43(12): 1182-91
Article in English | IMSEAR | ID: sea-59161
ABSTRACT
The study was undertaken to analyze the rate of uptake and utilization of various amino acids by Azospirillum brasilense Sp81 (RG) in a basal mineral salts solution under non-nitrogen fixing condition. These amino acids including other nitrogenous compounds were tested for both N- and C-sources. The kinetic constants (Km and Vmax) of uptake of some amino acids (e.g. lysine, arginine, proline, glutamine and glutamic acid) were exploited using a Hanes-Woolf plot, and discussed in the context of nitrogen starvation or both carbon and nitrogen starvation. To summarize all the kinetic data for these amino acids strongly suggested that the mode of these amino acids utilization in this bacterium followed the same general pattern, although the quantitative differences were there. A single amino acid was able to satisfy the nitrogen needs of this bacterium in basal mineral salts solution, and this possibility could be considered for the cost-effective growth medium for this bacterium in the biotechnological industry.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Carbon / Kinetics / Azospirillum brasilense / Growth Substances / Amino Acids Country/Region as subject: South America / Brazil Language: English Journal: Indian J Exp Biol Year: 2005 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Carbon / Kinetics / Azospirillum brasilense / Growth Substances / Amino Acids Country/Region as subject: South America / Brazil Language: English Journal: Indian J Exp Biol Year: 2005 Type: Article