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Analogue-resistant mutants of Azotobacter chroococcum derepressed for nitrogenase activity and early ammonia excretion having potential as inoculants for cereal crops.
Indian J Exp Biol ; 2000 Apr; 38(4): 373-8
Article in English | IMSEAR | ID: sea-59195
ABSTRACT
Spontaneous mutants resistant to methionine sulfoximine (Msx), methyl alanine (Mal) and methyl ammonium chloride (Mac) were derived from A. chroococcum strain A103. Msx and Mal-resistant mutants expressed 1.73 to 10.98% of the fully derepressed nitrogenase activity when grown in Burk's medium containing ammonium acetate. Mac-resistant mutants did not express nitrogenase activity in ammonium acetate supplemented medium. The mutants excreted ammonia even after 2 days of growth and some mutants excreted more ammonia as compared to the parent. Selected mutants were inoculated on wheat (Triticum aestivum) and barley (Hordeum vulgare) under field conditions. Majority of the derepressed mutants increased grain yield of wheat and barley varying from 1.2 to 33.3%. However, host-dependent effects on grain yield were observed with different mutants. Two mutants, Mal 27 and Mac 19 showed significant increase in grain yields of both the crops. The results suggest that metabolic analogue-resistant mutants of Azotobacter have potential for use as a biofertilizer for cereal crops.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Azotobacter / Drug Resistance, Microbial / Edible Grain / Alanine / Ammonia / Methionine Sulfoximine / Methylamines / Mutation / Nitrogen Fixation / Nitrogenase Language: English Journal: Indian J Exp Biol Year: 2000 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Azotobacter / Drug Resistance, Microbial / Edible Grain / Alanine / Ammonia / Methionine Sulfoximine / Methylamines / Mutation / Nitrogen Fixation / Nitrogenase Language: English Journal: Indian J Exp Biol Year: 2000 Type: Article