Isolation and subcloning of chitinase clone from chickpea genomic library.
Indian J Exp Biol
;
1996 Jun; 34(6): 594-6
Article
in English
| IMSEAR
| ID: sea-59218
ABSTRACT
Chickpea genomic library constructed earlier in phage lambda (EMBL-3) was screened for the presence of chitinase clone using tobacco chitinase cDNA as a probe. Positive clones obtained by primary screening of plaques (2 x 10(6)) were ascertained by secondary and tertiary screening. Presence of chitinase insert in the positive clones obtained, was further confirmed by restricting phage DNA with Sal I and then doing southern with tobacco chitinase. The insert band was eluted out and subcloned in puc 19 plasmid.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plants, Medicinal
/
Genomic Library
/
Chitinases
/
Cloning, Molecular
/
Genome, Plant
/
Fabaceae
Language:
English
Journal:
Indian J Exp Biol
Year:
1996
Type:
Article
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