Bioreactor operated production of lipase: castor oil hydrolysis using partially-purified lipase.
Indian J Exp Biol
;
1999 May; 37(5): 481-6
Article
in English
| IMSEAR
| ID: sea-59251
ABSTRACT
A highly stable lipase from Pseudomonas aeruginosa KKA-5 was produced by batch cultivation technique employing shake flask and 5 L-bioreactor. The bioreactor was run at different airflow rates. Low airflow rates (1 and 3 L/min), did not lead to effective growth and lipase production. Growth increased by about one order and lipase production increased by about 6 times, at an airflow rate of 5 L/min. Lipase production occurred during decelerated cell growth. A highly stable lipase was produced which retained its activity in the running bioreactor, even after a period of one month. This stable lipase was partially-purified using ammonium sulphate precipitation technique. Castor oil was hydrolyzed using 300U crude and partially-purified lipase, each. Approximately 21-fold, partially-purified lipase could hydrolyze 81% castor oil within a period of 96 hr, where as only 63% hydrolysis was obtained, in 216 hour, when crude lipase was used.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Pseudomonas aeruginosa
/
Castor Oil
/
Bioreactors
/
Hydrolysis
/
Lipase
Language:
English
Journal:
Indian J Exp Biol
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS