Studies on cytochrome oxidase in carbon tetrachloride treated rats.
Indian J Exp Biol
;
1999 Nov; 37(11): 1139-41
Article
in English
| IMSEAR
| ID: sea-59349
ABSTRACT
Cytochrome c oxidase was purified from control and CCl4 treated rats and its kinetic properties were studied. The activity of the enzyme was inhibited by 51% in CCl4 (4 g per kg body weight for 24 hr) treated rats. Studies on the kinetic properties showed that the K(m) of the enzyme increased by 60% while Vmax decreased by 44% in CCl4 treated rats compared to controls. The content of cytochrome aa3 was decreased by 34% while cytochrome b and c were not affected by CCl4 treatment. Phosphatidylcholine, phosphatidylethanolamine and cardiolipin were decreased significantly by 40%, 49% and 60% respectively in CCl4 treated rats. A decrease in the cytochrome aa3 content and a change in the lipid environment of the membrane are probably responsible for a decreased rate of electron transfer from cytochrome c to oxygen.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Male
/
Mitochondria, Liver
/
Carbon Tetrachloride
/
Kinetics
/
Rats, Wistar
/
Electron Transport Complex IV
/
Animals
Language:
English
Journal:
Indian J Exp Biol
Year:
1999
Type:
Article
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