Direct somatic embryogenesis and transformation in Cicer arietinum L.
Indian J Exp Biol
;
1996 Jul; 34(7): 716-8
Article
in English
| IMSEAR
| ID: sea-59480
ABSTRACT
Somatic embryos were induced directly from immature cotyledons of the genotype of chickpea ICC 4918 (annigiri) on B5 medium supplemented with 2,4,5-T or 2,4-D in combination with BA or KN. Successful transformation was achieved via somatic embryogenesis using Agrobacterium tumefaciens strain LBA4404, carrying a binary plasmid vector system containing neomycin phosphotransferase (NPT II) gene as the selectable marker and beta-glucuronidase (GUS) as a reporter gene. Histochemical staining for GUS expression was observed as primary evidence for transformation.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plants, Medicinal
/
Transformation, Genetic
/
Gene Transfer Techniques
/
Histocytochemistry
Language:
English
Journal:
Indian J Exp Biol
Year:
1996
Type:
Article
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