Paw oedema test for detection of Salmonella enterotoxin : modification and standardization.
Indian J Exp Biol
;
1990 Dec; 28(12): 1141-4
Article
in English
| IMSEAR
| ID: sea-59525
ABSTRACT
Paw oedema test (POT) was standardized with modifications for the detection of Salmonella enterotoxin. Instead of measuring the weight of the inoculated paws after amputating the limbs at 48 hr post-inoculation, percent relative thickness of the order of 121 +/- 3.8% at 24-48 hr was found to be a better index. This test yielded parallel results to rabbit ligated ileal loop (RLIL) technique. The test was positive with enterotoxic crude cell lysates (CL) and cell free-culture-supernatants (CFCS) of S. newport and S. typhimurium, partially purified and purified enterotoxin of S. newport and purified cholera toxin. The test was found to be specific in that non-enterotoxic CFCS did not cause significant increase in the thickness. Minimum detection level of purified S. newport enterotoxin was estimated to be as low as 20 micrograms. Thus, the modified POT was considered to be an effective and economical bioassay model for the detection of Salmonella enterotoxin.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Salmonella
/
Bacterial Toxins
/
Edema
/
Endotoxins
/
Enterotoxins
/
Foot
/
Animals
/
Mice
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1990
Type:
Article
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