Purification of Salmonella stanley enterotoxin and its immunology and dermatotoxicity.
Indian J Exp Biol
;
1992 Jan; 30(1): 23-5
Article
in English
| IMSEAR
| ID: sea-59544
ABSTRACT
Isolation of an enterotoxic factor from cell-free-culture-supernatant of S. stanley was achieved to homogeneity using salt precipitation, dialysis and molecular seive chromatography through Sephadex G-100 and G-200 columns. The purified enterotoxic factor yielded a single protein band on polyacrylamide gel electrophoresis, induced antibodies in the rabbit and showed single band on agar gel precipitation. It induced fluid accumulation in the rabbit ligated ileal loop (RLIL) and was neutralized by the homologous antiserum. Antigenically it was not related to cholera toxin but with enterotoxin of other Salmonella serotypes. It also exerted dermatotoxic effect in the rabbit skin causing marked central necrosis with peripheral erythema.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Salmonella
/
Skin
/
Enterotoxins
/
Erythema
/
Necrosis
Language:
English
Journal:
Indian J Exp Biol
Year:
1992
Type:
Article
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