In vitro formation of organ specific proximate carcinogen of benzo(a)pyrene by rat homogenates.
Indian J Exp Biol
;
2002 Sep; 40(9): 1067-70
Article
in English
| IMSEAR
| ID: sea-59560
ABSTRACT
In order to determine the organ specific carcinogenicity of benzo(a)pyrene (B(a)P), its metabolites, formed in vitro by incubation with the homogenates from liver, lungs, kidneys, intestine and brain of rats, were isolated by TLC and spectroscopy. B(a)P was found to be converted into a number of metabolites by different tissue homogenates. The results showed that the proximate carcinogenic metabolite, 7,8-dihydro-7,8-dihydroxy B(a)P was formed only when rat lung and kidney homogenates were incubated with B(a)P in vitro. The UV spectral analysis also confirmed the formation of this metabolite only on incubation of B(a)P with rat lung and kidney homogenates. As the proximate carcinogenic metabolite was only formed by incubating B(a)P with the homogenates from target organs, its organ specific carcinogenicity may be explained.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Organ Specificity
/
Rats
/
Spectrophotometry, Ultraviolet
/
Benzo(a)pyrene
/
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
/
Brain
/
Carcinogens
/
Chromatography, Thin Layer
/
Rats, Wistar
/
Intestines
Language:
English
Journal:
Indian J Exp Biol
Year:
2002
Type:
Article
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