Relative superiority of c-ELISA for detection of bluetongue virus antibodies.
Indian J Exp Biol
;
1995 Nov; 33(11): 880-2
Article
in English
| IMSEAR
| ID: sea-59672
ABSTRACT
Anti-VP7 bluetongue virus (BTV) antibodies were detected in sera of ruminant livestock using competitive enzyme linked immunosorbent assay (c-ELISA). The per cent positivity for BTV antibodies recorded in sheep was 34.61% in Haryana, 7.14% in Himachal Pradesh and 28.88% in Punjab. Goat showed 64% positivity in Haryana, 67.34% in Himachal Pradesh and 88.33% in Rajasthan whereas cattle showed 67.44% positivity in Haryana, 52.17% in Punjab and 59.15% in Rajasthan for BTV antibodies. The percent positivity recorded for buffalo was 54.05% in Punjab. A comparison of c-ELISA with dot-ELISA for a total of 181 serum samples of sheep and cattle was also done. The performance of c-ELISA relative to dot-ELISA was found to have a sensitivity of 84.61% and specificity of 89.32%.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Buffaloes
/
Cattle
/
Enzyme-Linked Immunosorbent Assay
/
Goats
/
Sheep
/
Sensitivity and Specificity
/
Bluetongue
/
Bluetongue virus
/
Animals
/
Antibodies, Viral
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1995
Type:
Article
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