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Effect of gamma irradiation on chemical and biological properties of lipopolysaccharide from Salmonella typhimurium.
Indian J Exp Biol ; 1998 Jun; 36(6): 588-92
Article in English | IMSEAR | ID: sea-59701
ABSTRACT
Lipopolysaccharide (LPS) from S. typhimurium on exposure to gamma-radiation resulted in decrease in toxicity and was less mitogenic, Silver stained profiles of irradiated LPS on polyacrylamide gels revealed complete loss of its heteropolysaccharides which was confirmed further by analysing lipid A and LPS from Salmonella minnesota Re mutants on SDS-PAGE. Glucosamine and 2-keto 3-deoxy-octonate(Kdo) contents were significantly decreased on treatment. Lipid A obtained by removal of heteropolysaccharides from LPS was less toxic on exposure to gamma radiations.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Salmonella typhimurium / Spleen / Lipopolysaccharides / Gamma Rays / Animals / Mice / Mice, Inbred C57BL / Mitogens Language: English Journal: Indian J Exp Biol Year: 1998 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Salmonella typhimurium / Spleen / Lipopolysaccharides / Gamma Rays / Animals / Mice / Mice, Inbred C57BL / Mitogens Language: English Journal: Indian J Exp Biol Year: 1998 Type: Article