ELISA for detection of dengue virus-induced cytokine and its antibody.
Indian J Exp Biol
;
1997 Mar; 35(3): 225-31
Article
in English
| IMSEAR
| ID: sea-59794
ABSTRACT
ELISA technique has been standardized for detection of a dengue type 2 virus (DV)-induced cytokine, the cytotoxic factor (CF) and CF-specific antibodies. The performed ELISA was found to be sensitive (90.9%), specific (92.5%), accurate (91%) and reproducible and was able to detect a minimum of 7 ng/ml CF in the test samples. The technique was used to detect CF in DV inoculated mouse sera and DV-infected mouse spleen cell culture fluids. Significant utility of the test was detection of a CF-like cytokine in the sera of human cases of dengue haemorrhagic fever.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Cytokines
/
Dengue Virus
/
Animals
/
Mice
/
Antibodies, Viral
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1997
Type:
Article
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