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ELISA for detection of dengue virus-induced cytokine and its antibody.
Indian J Exp Biol ; 1997 Mar; 35(3): 225-31
Article in English | IMSEAR | ID: sea-59794
ABSTRACT
ELISA technique has been standardized for detection of a dengue type 2 virus (DV)-induced cytokine, the cytotoxic factor (CF) and CF-specific antibodies. The performed ELISA was found to be sensitive (90.9%), specific (92.5%), accurate (91%) and reproducible and was able to detect a minimum of 7 ng/ml CF in the test samples. The technique was used to detect CF in DV inoculated mouse sera and DV-infected mouse spleen cell culture fluids. Significant utility of the test was detection of a CF-like cytokine in the sera of human cases of dengue haemorrhagic fever.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Humans / Enzyme-Linked Immunosorbent Assay / Cytokines / Dengue Virus / Animals / Mice / Antibodies, Viral Type of study: Diagnostic study Language: English Journal: Indian J Exp Biol Year: 1997 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Humans / Enzyme-Linked Immunosorbent Assay / Cytokines / Dengue Virus / Animals / Mice / Antibodies, Viral Type of study: Diagnostic study Language: English Journal: Indian J Exp Biol Year: 1997 Type: Article