Detection of px gene product of bovine leukemia virus in infected cells.
Indian J Exp Biol
;
1990 Dec; 28(12): 1107-11
Article
in English
| IMSEAR
| ID: sea-59934
ABSTRACT
Bovine leukemia virus (BLV), like its closest relatives human T-cell leukemia virus-I and II, contain a 'px' gene, between the 'env' gene and the 3' long terminal repeat in its genome. A monoclonal antibody prepared against a synthetic oligopeptide whose sequence was deduced from highly conserved region of 'px' gene of BLV, was used to detect the presence of 'px' gene product in chronically BLV infected synchronised cells. By immunoperoxidase staining the 'px' gene product was detected maximum after 6-9 hr after synchronization in the nucleus of the cells which demonstrated the close interaction of it with viral DNA which is integrated with host cell genome.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Viral Proteins
/
Molecular Sequence Data
/
Retroviridae Proteins, Oncogenic
/
Cells, Cultured
/
Gene Products, tax
/
Genes, pX
/
Amino Acid Sequence
/
Leukemia Virus, Bovine
/
Animals
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1990
Type:
Article
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