Association of cucumovirus and potyvirus with betelvine (Piper betle L.) as evidenced by ELISA and RT-PCR.
Indian J Exp Biol
;
2003 Mar; 41(3): 273-6
Article
in English
| IMSEAR
| ID: sea-60032
ABSTRACT
An attempt was made to detect various viruses of Piper betle grown at Mahoba and Banthara in India. DAC-ELISA and RT-PCR tests were performed in leaf sap samples of betelvine for detection of a cucumovirus (Cucumber mosaic virus) and potyvirus (Bean yellow mosaic virus) using specific antibodies and universal primers of respective viruses. DAC-ELISA could detect only CMV. However, RT-PCR detected both cucumovirus and potyvirus infection in betelvine samples. Association of CMV with betelvine was observed for the first time in the present study.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
DNA, Viral
/
Enzyme-Linked Immunosorbent Assay
/
Base Sequence
/
Potyvirus
/
DNA Primers
/
Cucumovirus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Antibodies, Viral
Language:
English
Journal:
Indian J Exp Biol
Year:
2003
Type:
Article
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