Comparative evaluation of sensitivity of RNA-polyacrylamide gel electrophoresis and dot immunobinding assay for detection of bluetongue virus in cell culture.
Indian J Exp Biol
;
1999 Feb; 37(2): 157-60
Article
in English
| IMSEAR
| ID: sea-60105
ABSTRACT
Bluetongue virus serotype 1 (Avikanagar isolate) was grown in BHK-21 cell line and titrated. The titre of the virus in BHK-21 cell line was 10(6) TCID50/ml. RNA-polyacrylamide gel electrophoresis (RNA-PAGE) and dot immunobinding assay (DIA) were performed on 10-fold serial dilutions of the sonicated cell culture material. The results indicated that the minimum limit of detection of the virus by RNA-PAGE and DIA was 10(5) TCID50/ml.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
RNA, Viral
/
Immunoblotting
/
Cell Line
/
Sensitivity and Specificity
/
Cricetinae
/
Bluetongue virus
/
Electrophoresis, Polyacrylamide Gel
/
Animals
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1999
Type:
Article
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