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Effects of oxidizing and reducing agents on ovine pulmonary artery responses to nitric oxide donors, sodium nitroprusside and 3-morpholino-sydnonimine.
Indian J Exp Biol ; 2006 Dec; 44(12): 964-70
Article in English | IMSEAR | ID: sea-60225
ABSTRACT
Nitrovasodilators-sodium nitroprusside (SNP; 10(-9)-10(-4) M) and 3-morpholino-sydnonimine (SIN-1; 10(-9)-10(-4) M) produced concentration-dependent relaxation of the fourth generation sheep pulmonary artery, preconstricted with 5-hydroxytryptamine (1 microM). Oxidizing agents [oxidized glutathione (GSSG, 1 mM) and CuSO4 (5 and 20 microM)] and reducing agents [dithiothreitol (DTT, 0.1 mM), ascorbic acid (1 mM) and reduced glutathione (GSH, 1 mM)] caused opposite effects on nitric oxide (NO)-induced vasodilation in the artery. Ascorbic acid and GSH potentiated the NO responses, while GSSG and CuSO4 inhibited relaxation caused by the nitrovasodilators. DTT, however, reduced the relaxant potency and efficacy of SNP and SIN-1. Pretreatment of the pulmonary artery strips with DTT (0.1 mM) inhibited SNP (10 microM)-induced Na(+)-K(+)-ATPase activity, while ascorbic acid (1 mM) and GSH (1 mM) had no effect either on basal or SNP (10 microM)-stimulated 86Rb uptake, an index of Na(+)-K(+)-ATPase activity, in ovine pulmonary artery. The results suggest that reducing agents like ascorbic acid may have beneficial effect in improving the vascular function under oxidative stress.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Pulmonary Artery / Vasodilation / Molsidomine / Nitroprusside / Sheep / Oxidants / Reducing Agents / Nitric Oxide Donors / Animals Language: English Journal: Indian J Exp Biol Year: 2006 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Pulmonary Artery / Vasodilation / Molsidomine / Nitroprusside / Sheep / Oxidants / Reducing Agents / Nitric Oxide Donors / Animals Language: English Journal: Indian J Exp Biol Year: 2006 Type: Article