Evidence for integration of IS1 and IS10 in recombinant plasmid containing rearrangement hot-spot(rhs) common-shared block(CSB) in Escherichia coli K-12.

ABSTRACT

The integration of IS1 and IS10 was reported in the recombinant plasmid containing the 3070 bp rearrangement hotspot(rhs) common-shared block(CSB) in Escherichia coli K-12. The integration of IS1 was found to be in rhs(CSB) portion, whereas the integration of IS10 was found to be in both rhs(CSB) and vector portions. The bacterial cells containing the recombinant plasmid grew very slowly. But the integration of IS1 or IS10 in rhs(CSB) portion made the host grow rapidly and overgrew the slow-growing population inheriting the recombinant plasmids without IS-sequences. The sites of integration of IS1 and IS10 were different as was judged from restriction endonuclease mapping. These are rare examples of interchromosomal mobilisation of IS1 and IS10 from host chromosome into plasmid.