Fed-batch fermentation studies with Bacillus thuringiensis H-14 synthesising endotoxin.
Indian J Exp Biol
;
1991 Nov; 29(11): 1031-4
Article
in English
| IMSEAR
| ID: sea-60289
ABSTRACT
Cell yield and toxicity of B. thuringiensis H-14 was improved markedly by adopting a simple fed-batch fermentation technique based on controlling glucose concentration. Maintenance of steady glucose concentration (0.3-0.5%) in the culture medium was achieved by the continuous addition of concentrated glucose solution. Addition of glucose at 3 g/hr/l of culture starting from 3rd hr till 16th hr of fermentation was found to yield cell densities of 80 g/l (wet weight) which represented a nearly 3-fold increase over the batch mode. A fivefold increase in toxicity was obtained by fed-batch fermentation. Cultivation of B. thuringiensis H-14 to high cell densities had no negative effect on sporulation and toxin synthesis. The rate of pH drop and dissolved oxygen level were within manageable limits.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacillus thuringiensis
/
Bacterial Proteins
/
Bacterial Toxins
/
Endotoxins
/
Fermentation
/
Hemolysin Proteins
Language:
English
Journal:
Indian J Exp Biol
Year:
1991
Type:
Article
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