In vitro control of fasciation in proliferating nucellar embryos of Mangifera indica L. var totapari red small for cloning.
Indian J Exp Biol
;
2003 Nov; 41(11): 1311-6
Article
in English
| IMSEAR
| ID: sea-60403
ABSTRACT
Nucellar tissue contained in ovular halves of young fruits of Mangifera indica L. totapari red small, a dwarfing rootstock, differentiated fasciated embryonal structures in presence of 6-benzylaminopurine [BAP(0.15 mg l(-1))], 6-(gamma-gamma-dimethylallylamino) purine [2iP(0.15 mg l(-1))] and indole-3-acetic acid [(IAA(0.5 mg l(-1))] incorporated in the semisolid medium during 50-60 days. Due to embryonal fasciation, hardly 2-3 well-formed embryos could be obtained per culture of proliferating embryos. Of the 3 ethylene inhibitors [L-alpha-(2-aminoethoxyvinyl)-glycine-HCl (AVG), AgNO3 and salicylic acid (SA)] used, embryonal fasciation and necrosis of intervening tissue was completely controlled by 3-4 subcultures of fasciated mass of embryos under the influence of AVG (0.05 mg l(-1)) in presence of adenine sulphate [AdS (50 mg l(-1))] incorporated in the same medium. Almost synchronized development of isolated embryos, measuring ca 2 cm in length, was observed in a different medium used in liquid stationary state and supplemented, particularly with stress-producing substances [abscisic acid (ABA, 0.01 mg l(-1)); and polyethylene glycol (PEG, 100 mg l(-1))] besides certain other modifications. About 34% convertibility of processed embryos was obtained during a period of 90 days. The plantlets had well-developed roots along with laterals which were longer than leafy shoots. In vitro raised plants survived ex vitro for about 2 months.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Growth Regulators
/
Polyethylene Glycols
/
Purines
/
Regeneration
/
Seeds
/
Surface-Active Agents
/
Adenine
/
Abscisic Acid
/
Germination
/
Mangifera
Language:
English
Journal:
Indian J Exp Biol
Year:
2003
Type:
Article
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