Immunological identification of two female-specific proteins from the plasma of Indian freshwater murrel, Channa punctatus (Bloch).
Indian J Exp Biol
;
2002 Mar; 40(3): 288-95
Article
in English
| IMSEAR
| ID: sea-60418
ABSTRACT
Existence of a non-phosphorylated female-specific protein (FS II), in addition to phosphorylated vitellogenin (FS I), in the plasma of murrel by exogenous administration of estradiol-17beta is reported. Polyspecific rabbit antibodies were raised against estrogen-inducible murrel plasma proteins. This antiserum was absorbed with normal male serum in order to obtain female-specific antiserum (FSAS). Radial immunodiffusion studies suggested that both the proteins (FS I and FS II) were present in the plasma of E2-treated and normal vitellogenic females and in the ovarian homogenate from gravid females, but absent in normal male plasma. Autoradiographic experiments demonstrated that phosphorus moiety was attached with FS I only. Further, immunoelectrophoretic analysis and peptide maps supported the observation that FS I and FS II were discrete, unrelated female-specific proteins.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Perciformes
/
Female
/
Male
/
Blood Proteins
/
Immunochemistry
/
Vitellogenins
/
Sex Characteristics
/
Animals
/
Antibody Specificity
Type of study:
Prognostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
2002
Type:
Article
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