Influence of alpha-tocopherol on doxorubicin-induced lipid peroxidation, swelling and thiol depletion in rat heart mitochondria.

ABSTRACT

Effect of alpha-tocopherol on doxorubicin-induced swelling in rat heart mitochondria was studied in vitro. Mitochondria was isolated from control and alpha-tocopherol treated rats. Various concentrations of doxorubicin were added to mitochondrial suspension. Swelling, lipid peroxidation and thiol depletion were measured. Concentration and time dependent increase in swelling was noted with increase in lipid peroxidation and thiol depletion in mitochondria isolated from control rats. In alpha-tocopherol treatment, thiol depletion is significantly prevented with reduced lipid peroxidation and swelling.