Survival of Shigella dysenteriae type 1 on fomites.
J Health Popul Nutr
;
2001 Sep; 19(3): 177-82
Article
in English
| IMSEAR
| ID: sea-605
ABSTRACT
Studies have shown that various objects, such as utensils, toys, and clothes, can serve as vehicles for transmission of Shigella spp. Shigellae can become viable but non-culturable (VBNC) when exposed to various environmental conditions as shown in earlier studies. The present study was carried out to detect VBNC Shigella dysenteriae type 1 on various fomites by direct viable counting, polymerase chain reaction (PCR), and fluorescent antibody methods. S. dysenteriae type 1 was inoculated onto cloth, wood, plastic, aluminum, and glass objects. Results showed that 1.5-4.0 hours after inoculation, S. dysenteriae type 1 became non-culturable, and after five days, non-culturable but viable S. dysenteriae type 1 could be detected by both PCR and fluorescent antibody techniques. Fomites can be considered an important potential route of transmission of VBNC S. dysenteriae type 1 and a significant factor in the epidemiology of shigellosis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Shigella dysenteriae
/
Bangladesh
/
Humans
/
Disease Reservoirs
/
Colony Count, Microbial
/
Polymerase Chain Reaction
/
Fluorescent Antibody Technique
/
Dysentery, Bacillary
Country/Region as subject:
Asia
Language:
English
Journal:
J Health Popul Nutr
Journal subject:
Gastroenterology
/
Nutritional Sciences
/
Public Health
Year:
2001
Type:
Article
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