Characteristics of monoclonal antibodies against porcine zona pellucida-3 and their functional relevance.
Indian J Exp Biol
;
1992 Nov; 30(11): 1000-5
Article
in English
| IMSEAR
| ID: sea-60696
ABSTRACT
Seven monoclonal antibodies (MAs) against 55 kDa glycoprotein family of porcine zona pellucida (ZP3) reacting with either ZP3 alpha (MA-7, MA-27, MA-28) or ZP3 beta (MA-1, MA-2, MA-11, MA-30) have been described. MA-1, -2, -27, -28 and -30 do not recognize carbohydrate determinants as shown by their reactivity to the deglycosylated (DG) ZP3 alpha and ZP3 beta. Indirect immunoperoxidase studies showed that all MAs reacted with zona pellucida from porcine and monkey ovaries. Only MA-1 and -27 reacted with ZP from rabbit ovary as well, while none of the MAs recognised mouse ZP, MA-7, -11, -27, -28 and -30 inhibited in vitro, the zona lysis by trypsin as well as the binding of ZP3 to sperm membrane vesicle as investigated by ELISA.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Ovary
/
Rabbits
/
Sperm-Ovum Interactions
/
Spermatozoa
/
Spleen
/
Swine
/
Zona Pellucida
/
Female
/
Male
/
Membrane Glycoproteins
Language:
English
Journal:
Indian J Exp Biol
Year:
1992
Type:
Article
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