In vitro mass multiplication of Ophiorrhiza mungo Linn.
Indian J Exp Biol
;
2004 Jun; 42(6): 639-42
Article
in English
| IMSEAR
| ID: sea-60740
ABSTRACT
A protocol for in vitro mass multiplication of plants through seedling (shoot) cultures was established for Ophiorrhiza mungo. Maximum number of adventitious shoots per shoot culture (10.4 +/- 1.72) was initiated on MS solid medium supplemented with BAP (2.22 microM) after 3 weeks. Shoots were further multiplied (12.8 +/- 2.8) through subculture of intact shoots and reculture of nodal segments of aseptic shoots (6.5 +/- 0.94) in MS solid medium containing BAP (0.89 microM). Shoot elongation (1.27 +/- 0.12 cm) was achieved in the medium containing GA3 (1.44 microM) in two weeks. Rooting was favoured in basal agar medium supplemented with IBA (12.3 microM) plus NAA (1.07 microM). The plants were successfully established (100%) in the pots containing sand and top soil (11) mixture in a period of two weeks.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Growth Regulators
/
Time Factors
/
Cell Division
/
Silicon Dioxide
/
Plant Shoots
/
Plant Roots
/
Plant Physiological Phenomena
/
Rubiaceae
/
Culture Media
/
Agar
Language:
English
Journal:
Indian J Exp Biol
Year:
2004
Type:
Article
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