Cost effectiveness of colony lysis and colony PCR methods for screening of recombinant Escherichia coli colonies--a comparative study.
Indian J Exp Biol
;
2008 Oct; 46(10): 731-5
Article
in English
| IMSEAR
| ID: sea-60779
ABSTRACT
Economizing the research protocols by using low cost technologies is the need of laboratories of developing world. Screening of recombinant E. coli colonies is the crucial step in gene cloning and expression studies. In the present study, the cost effectiveness of colony lysis method and colony PCR method in the screening of recombinant E. coli colonies was compared. The colony lysis method was 20 two times more cost effective and less time consuming and can be used to screen the recombinant E. coli colonies in large scale instead of colony PCR method.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Recombination, Genetic
/
Polymerase Chain Reaction
/
Bacteriological Techniques
/
Genetic Techniques
/
Cost-Benefit Analysis
/
Escherichia coli
Type of study:
Diagnostic study
/
Health economic evaluation
/
Screening study
Language:
English
Journal:
Indian J Exp Biol
Year:
2008
Type:
Article
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