Partial cloning and sequencing of a cDNA encoding bonnet monkey (Macaca radiata) oviduct specific protein.

ABSTRACT

Based on the complete nucleic acid sequence of human estrogen dependent oviductal protein and deduced amino acid sequence, potential antigenic site of the protein was identified. Two oligonucleotide primers were designed to specifically amplify the region which includes this antigenic site. With the expectation that the human, and monkey oviductins would have high nucleotide sequence homology, Bonnet monkey oviduct along with endometrium was obtained on day 5, 9, 12 and 22 of the cycle. Using RT PCR correct sized PCR product was detected in oviduct taken from day 9 and 12 of the cycle. PCR product was cloned into pBluescript KS[+] and nucleic acid sequence determined. A 96% homology to human, baboon and rhesus monkey estrogen induced glycoprotein, and a 84-88% homology to other mammalian oviductal protein was noted, thus confirming the authenticity of cDNA clone for monkey fallopian tube specific protein.