Monoclonal antibodies against bovine growth hormone.
Indian J Exp Biol
;
1999 Jul; 37(7): 650-4
Article
in English
| IMSEAR
| ID: sea-61113
ABSTRACT
A number of mouse x mouse hybridomas producing monoclonal antibodies (MAbs) against bovine growth hormone (bGH) were prepared by fusion of spleen cells from bGH-primed mice (Balb/c) with non-secretory mouse myeloma cells (PAIOP3) and characterized. MAbs obtained from three fusion experiments belonged to IgM, IgG1 and IgG2b class/subclass of antibodies. Cross-reaction studies indicated that generated antibodies were against three different epitopes of bGH. VIA6E8 (IgG1) and VIIB2E11C9 (IgM) did not cross-react with ovine prolactin (oPRL), ovine leutinizing hormone (oLH) and porcine follicle stimulating hormone. Antibody VIB3C9E8 (IgM) exhibited cross-reaction with oPRL and oLH. Antibody VIC1F9 (IgG2b) cross reacted with oPRL. All MAbs were against conformational epitopes of bGH.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Cattle
/
Growth Hormone
/
Tumor Cells, Cultured
/
Cross Reactions
/
Animals
/
Mice
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
Language:
English
Journal:
Indian J Exp Biol
Year:
1999
Type:
Article
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