Effect of high-fat diet on mice intestinal brush border membrane composition.
Indian J Exp Biol
;
1993 Jun; 31(6): 536-9
Article
in English
| IMSEAR
| ID: sea-61148
ABSTRACT
Effect of feeding high-fat (26% fat) diet to mice for 21 days on intestinal brush border membrane composition was evaluated by comparing with controls fed 10% fat diet. 125I-labelled lectin binding and chemical analysis of fucose, sialic acid, hexoses and hexosamines revealed essentially similar results in control and test groups. Membrane phospholipids, expressed on dry membrane basis, were significantly reduced while total cholesterol was enhanced in experimental group compared to controls. Triglyceride content was not altered under these conditions. [14C]-acetate incorporation studies showed that decrease in phospholipid content was due to reduced synthesis of phospholipid constituents, in particular, phosphatidylcholine and phosphatidylethanolamine, as a result of feeding high fat diet. The results suggest that high amount of fat in the diet of adult mice does not alter sugar content in brush border membrane but affects membrane lipid composition.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phospholipids
/
Male
/
Carbohydrates
/
Molecular Sequence Data
/
Dietary Fats
/
Cell Membrane
/
Carbohydrate Sequence
/
Intestine, Small
/
Animals
/
Membrane Lipids
Language:
English
Journal:
Indian J Exp Biol
Year:
1993
Type:
Article
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