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Characterization of primed lymphocytes on the basic of sensitivity of chromatin to DNase I.
Indian J Exp Biol ; 1997 Nov; 35(11): 1156-60
Article in English | IMSEAR | ID: sea-61149
ABSTRACT
Certain qualitative criteria for primed lymphocytes in the expression of cytotoxic function have been studied. Unlike normal lymphocytes, primed lymphocytes expressed cytotoxicity even when DNA synthesis and new gene expression were inhibited by hydroxyurea (HU) and bromodeoxyuridine (BU) respectively. Such differential cytotoxic expression in presence of HU and BU by primed lymphocytes might have their basis in conformational change within the chromatin. Chromatin from primed lymphocytes was more susceptible to DNase I digestion than virgin lymphocytes indicating exposition of more DNase I sensitive sites in primed state. The result suggest the presence of more ready to act sites for the polymerases in the genomic material of primed lymphocytes even at quiescent state.
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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Bromodeoxyuridine / Chromatin / Lymphocytes / Nucleic Acid Synthesis Inhibitors / Deoxyribonuclease I / Hydroxyurea / Animals / Mice / Antimetabolites Type of study: Qualitative research Language: English Journal: Indian J Exp Biol Year: 1997 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Bromodeoxyuridine / Chromatin / Lymphocytes / Nucleic Acid Synthesis Inhibitors / Deoxyribonuclease I / Hydroxyurea / Animals / Mice / Antimetabolites Type of study: Qualitative research Language: English Journal: Indian J Exp Biol Year: 1997 Type: Article