Karyotype, Ag-NOR, CMA3 and SEM studies in a fish (Mystus tengara, Bagridae) with indication of female heterogamety.
Indian J Exp Biol
;
2003 Jun; 41(6): 603-8
Article
in English
| IMSEAR
| ID: sea-61169
ABSTRACT
Somatic karyotypes in M. tengara contained 54 chromosomes, comprising 26 homomorphic pairs in both sexes and one pair of heteromorphic nature in female (one big submetacentric and one small subtelocentric chromosomes), while in males this pair was homomorphic (with two big sub-metacentric chromosomes). The Nucleolus Organizer Regions (NORs) were located at one arm of the suspected sex elements in both sexes, while another pair of metacentric chromosomes (No.7) also showed Ag-positive arm. The CMA3 technique revealed relatively bright fluorescing zones in the regions of chromosomes that showed Ag-positive staining, revealing thereby the preponderance of GC-rich active sites of rRNA genes in NORs. SEM studies revealed clear heteromorphism to exist in the elements suspected as sex chromosomes in females.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Silver Nitrate
/
Female
/
Male
/
Microscopy, Electron, Scanning
/
Chromomycin A3
/
Fishes
/
Karyotyping
/
Animals
/
Nucleolus Organizer Region
Language:
English
Journal:
Indian J Exp Biol
Year:
2003
Type:
Article
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