Effect of theophylline on binding of delta-9-tetrahydrocannabinol with mammalian neuronal and non-neuronal membranes.
Indian J Exp Biol
;
1993 Feb; 31(2): 130-5
Article
in English
| IMSEAR
| ID: sea-61574
ABSTRACT
Delta-9-tetrahydrocannabinol (THC) (1.6 x 10(-6)-13.33 x 10(-6) M) binds to neuronal and non-neuronal subcellular membranes in a biphasic manner. Its binding to neuronal membranes occur in the following order synaptosome > myelin > brain microsome and brain mitochondria. Unlike brain membranes binding of delta-9-THC is greater with liver microsome than liver mitochondria. Irrespective of membranes theophylline (Th) (11.0 x 10(-6) and 550.0 x 10(-6) M) increases the binding of delta-9-THC by exposure of more number of delta-9-THC binding sites on the subcellular membranes without affecting its binding affinity. Failure of Th (11.0 x 10(-6)-550.0 x 10(-6) M) to increase the binding of delta-9-THC (1.6 x 10(-6)-13.33 x 10(-6) M) in solubilized membranes suggests the involvement of membrane lipid in the Th-induced enhancement of delta-9-THC binding.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Dronabinol
/
Theophylline
/
Brain
/
Male
/
Intracellular Membranes
/
Liver
/
Animals
/
Neurons
Language:
English
Journal:
Indian J Exp Biol
Year:
1993
Type:
Article
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