Comparative diagnostic potentiality of ELISA and dot-ELISA in prepatent diagnosis of experimental Fasciola gigantica infection in cattle.
Indian J Exp Biol
;
2005 Jun; 43(6): 536-41
Article
in English
| IMSEAR
| ID: sea-61577
ABSTRACT
A glycoprotein (27 kDa) was isolated from crude somatic antigen of Fasciola gigantica by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect ELISA and in dot-ELISA formats. Although, anti-27 kDa antibodies could be detected after 3 weeks post infection (WPI) by dot - ELISA which was one week later than indirect ELISA. The test, dot-ELISA, was more convenient in field application. By the test (dot-ELISA) the infection could be equally detected in animals infected with 100, 200 and 300 metacercariae of F. gigantica with high sensitivity. Further, the antigen (27 kDa) was not found to react with goat sera infected with Paramphistomum epiclitum, which are giving strong reaction to homologous immature and mature fluke antigens of P. epiclitum.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rabbits
/
Sepharose
/
Time Factors
/
Trematode Infections
/
Immunoglobulin G
/
Cattle
/
Enzyme-Linked Immunosorbent Assay
/
Goats
/
Antibodies, Helminth
/
Glycoproteins
Type of study:
Diagnostic study
/
Screening study
Language:
English
Journal:
Indian J Exp Biol
Year:
2005
Type:
Article
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