Microplate assay for detection of bacterial contamination in tissue culture media.
Indian J Exp Biol
;
1997 Jan; 35(1): 86-8
Article
in English
| IMSEAR
| ID: sea-61869
ABSTRACT
In the present study, a rapid and simple colorimetric technique has been described to determine the presence of bacteria in tissue culture medium used in animal cell culture. The microplate assay is based on utilization of MTT [3(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] by bacteria resulting in formation of formazan crystals which can be measured colorimetrically. Contaminated medium, a standard gram-negative and gram-positive bacteria produce formazan from MTT which is related to the bacterial load. The assay has utility in screening tissue culture reagents to detect the presence of bacteria.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Tetrazolium Salts
/
Thiazoles
/
Bacteria
/
Drug Contamination
/
Bacteriological Techniques
/
Crystallization
/
Culture Media
/
Formazans
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1997
Type:
Article
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