Heat modifiability of outer membrane protein of Pasteurella multocida serotype B:2.
Indian J Exp Biol
;
2002 Jan; 40(1): 106-8
Article
in English
| IMSEAR
| ID: sea-61892
ABSTRACT
Outer membrane proteins (OMP) are generally porins, functioning as molecular sieves assisting in the transmembrane transportation. Heat modifiable characteristics of OMP from P. multocida B 2 have been explored to know their basic characteristics on event of temperature rise. A major band of 32 kDa and two minor bands of approximately 39 and approximately 28 kDa were found to be heat modifiable. It is suggested that boiling at 100 degrees C in presence of beta mercaptoethanol for 5 min is sufficient for characterisation of OMP by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacterial Outer Membrane Proteins
/
Pasteurella multocida
/
Electrophoresis, Polyacrylamide Gel
/
Hot Temperature
/
Animals
/
Mice
Language:
English
Journal:
Indian J Exp Biol
Year:
2002
Type:
Article
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